Huang Guanqing, Tang Meilai, Wang Kaizhi, Liu Shihua (Surgical Department, the People's Hospital of Hunan)
A total of 108 animals were taken for study on the mechanism of action of the curative effect of electro-acupuncture in perforated peptic ulcer.
I. The effect of electroacupuncture on the anatomical relationship between the omentum and the site of an artificial perforation of the stomach in experimental animal was observed. Rabbits were fasted before study. An artificial perforation of the stomach, 0.6X0.6 cm{2} in size, 4-5 cm. proximal to the pylorus, was made after an exploratory laparotomy in each of 24 rabbits. Fluids were given parenterally for the first postoperative day. Zusanli point was acupunctured with a silver needle for 15 minutes, 4-6 times a day. Animals were killed and explored 24 hours postoperatively.
Result. In the acupuncture group, the omentum was found closely adherent to the gastric wound in 10 out of 12 animals free in only 2. While in the control group, only 4 out of 12 animals showed such adhesions and the remaining 8 showed no adhesion at all. (X{2}=6.1704, P<0.01)
II. The effect of acupuncture on the artificial gastric perforation was studied in 30 rabbits by means of scanning the movement of I labelled omentum towards the wound. Artificial gastric perforation and acupuncture treatment were performed in the same way as mentioned above. A tiny airtight plastic tube containing 6 mg of I (equivalent to 30-60 microcuries) was fixed just above the gastric wound and another to the lowermost edge of the omentum. All animals were scanned immediately after operation and scanning was repeated at 1,2,3,5 and 7 hours after electroacupuncture with a total of 6 readings for each animal.
Result: In 9 out of 15 animals in the treated group, the omentum moved toward the gastric wound and in 6 it did not, while in the control group, only 2 showed such movement of the omentum. (X{2}=7.032, P<0.01)
III. The effect of galvanized needling upon the absorbing power of the peritoneal cavity in experimental peritonitis was investigated in 30 rabbits. Two ml. of type "O" human blood were injected intraperitoneally in each animal. Half of them were electro-acupunctured 30 minutes later and the remaining half served as control. All were killed and explored 7 hours later and the peritoneal cavity was washed with 10 ml. of normal saline. The peritoneal fluid together with the residual blood was aspirated and its hemoglobin content was estimated with a type "72" photo-electric colorimeter. This served as a measure to evaluate the blood absorption power of the peritonium.
Result: The average level of hemoglobin in the treated group was 0.1163 while in the control group it was 0.3239. (T=5.1, P<0.01)
IV. The effect of electro-acupuncture on the diapedesis of white blood cells in experimental peritonitis was also studied in 22 guinea-pigs. 6 ml. of a mixture of equal amounts of brain and peptone were injected intraperitoneally to each animal. They were equally divided into two group: 11 were electro-acupunctured half an hour later and the remaining 11 served as control. All were killed 7 hours after needling and the abdomen was explored. The peritoneal fluid was smeared, stained and the white blood cells were differentially counted.
Result: In the needled animals, the average percentage of neutrophils was 80% and that of lymphocytes, 17%, while in the control group they were 60% and 34% respectively.
Conclusion:
1. Observations on the effect of electro-acupuncture for artificial gastric perforation in animals, using the technics exploratory laparotomy and of I scanning showed that electro-acupuncture promoted the movement of the omentum towards the wound, particularly in the early stage.
2. The fundamental pathological changes consisted of degeneration, exudation, absorption and proliferation. In the early stage of peritonitis, exudation and absorption prevailed. Further development was determined mainly by the severity of the infection and the body resistance.
3. Electro-acupuncture enhanced the absorption of blood in the peritoneal cavity.
4. The increase of neutrophils indicated that body resistance might be potentiated by electro-acupuncture.
A total of 108 animals were taken for study on the mechanism of action of the curative effect of electro-acupuncture in perforated peptic ulcer.
I. The effect of electroacupuncture on the anatomical relationship between the omentum and the site of an artificial perforation of the stomach in experimental animal was observed. Rabbits were fasted before study. An artificial perforation of the stomach, 0.6X0.6 cm{2} in size, 4-5 cm. proximal to the pylorus, was made after an exploratory laparotomy in each of 24 rabbits. Fluids were given parenterally for the first postoperative day. Zusanli point was acupunctured with a silver needle for 15 minutes, 4-6 times a day. Animals were killed and explored 24 hours postoperatively.
Result. In the acupuncture group, the omentum was found closely adherent to the gastric wound in 10 out of 12 animals free in only 2. While in the control group, only 4 out of 12 animals showed such adhesions and the remaining 8 showed no adhesion at all. (X{2}=6.1704, P<0.01)
II. The effect of acupuncture on the artificial gastric perforation was studied in 30 rabbits by means of scanning the movement of I labelled omentum towards the wound. Artificial gastric perforation and acupuncture treatment were performed in the same way as mentioned above. A tiny airtight plastic tube containing 6 mg of I (equivalent to 30-60 microcuries) was fixed just above the gastric wound and another to the lowermost edge of the omentum. All animals were scanned immediately after operation and scanning was repeated at 1,2,3,5 and 7 hours after electroacupuncture with a total of 6 readings for each animal.
Result: In 9 out of 15 animals in the treated group, the omentum moved toward the gastric wound and in 6 it did not, while in the control group, only 2 showed such movement of the omentum. (X{2}=7.032, P<0.01)
III. The effect of galvanized needling upon the absorbing power of the peritoneal cavity in experimental peritonitis was investigated in 30 rabbits. Two ml. of type "O" human blood were injected intraperitoneally in each animal. Half of them were electro-acupunctured 30 minutes later and the remaining half served as control. All were killed and explored 7 hours later and the peritoneal cavity was washed with 10 ml. of normal saline. The peritoneal fluid together with the residual blood was aspirated and its hemoglobin content was estimated with a type "72" photo-electric colorimeter. This served as a measure to evaluate the blood absorption power of the peritonium.
Result: The average level of hemoglobin in the treated group was 0.1163 while in the control group it was 0.3239. (T=5.1, P<0.01)
IV. The effect of electro-acupuncture on the diapedesis of white blood cells in experimental peritonitis was also studied in 22 guinea-pigs. 6 ml. of a mixture of equal amounts of brain and peptone were injected intraperitoneally to each animal. They were equally divided into two group: 11 were electro-acupunctured half an hour later and the remaining 11 served as control. All were killed 7 hours after needling and the abdomen was explored. The peritoneal fluid was smeared, stained and the white blood cells were differentially counted.
Result: In the needled animals, the average percentage of neutrophils was 80% and that of lymphocytes, 17%, while in the control group they were 60% and 34% respectively.
Conclusion:
1. Observations on the effect of electro-acupuncture for artificial gastric perforation in animals, using the technics exploratory laparotomy and of I scanning showed that electro-acupuncture promoted the movement of the omentum towards the wound, particularly in the early stage.
2. The fundamental pathological changes consisted of degeneration, exudation, absorption and proliferation. In the early stage of peritonitis, exudation and absorption prevailed. Further development was determined mainly by the severity of the infection and the body resistance.
3. Electro-acupuncture enhanced the absorption of blood in the peritoneal cavity.
4. The increase of neutrophils indicated that body resistance might be potentiated by electro-acupuncture.
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