2) Relationship between ChE-positive Nerves and the Peripheral Path of Conducting Needling Sensation
Department of Morphology, Institute of Acupuncture and Moxibustion, Academy of Traditional Chinese Medicine
91 male albino rats were used to observe ChE-positive nerve in somatic connective tissue and to study the peripheral path of conducting needling sensation relating to the acupuncture analgesia.
Acetylthiocholine or butylthiocholine was used as substrate, iso-OMPA was not added to the medium incubation to show the nerve near the resistance vessel by microscopy. Above of all, we observed some nerves in stretch preparation of subcutis which often accompany with arteriole and venule, and with their terminals branch into reticular glomerate and arborescent nerve endings to terminate in matrix of connective tissue. These nerves are named as ChE-positive nerves.
They are thin unmyelinated nerve fibers. After sympathetic surperior cervical ganglionectomy, they are survival. We have traced their whole course in association with small spinal nerve trunk in connective tissue. Therefore, the ChE-positive nerve is afferent through the somatic nerve.
We applied electro-acupuncture to subcutis of the points mimicking Xinming or/ and Huantiao on the both sides of the intact and the sympathetic ganglionectomy animals. Tissue specimens were taken from the points which elevate the pain threshold obviously during acupuncture analgesia.
The afferent receptor of acupuncture in the excised subcutis surounding the needles was searched by microscopy. The free nerve endings in several forms were discovered primarily. The ChE-positive nerves and their terminal branches were also observed in the group of sympathetic ganglionectomy. So we think that they are the path of conducting needling sensation during acupuncture analgesia.
In sarcolemma, muscular interconnective tissue, capsule of knee joint and skeletal muscle, there are ChE-positive nerve to terminate near the resistance vessels by several form of free nerve endings. According to the description of "Ling Shu", needling points is Huang membrane but not skin, skeleton or muscle. We think that somatic connective tissue might be a part of Huan membrane. In Huan membrane there are the structures conducting needling sensation.
According to the results of consecutive method whether added iso-OMPA or not to medium incubation, we divided the ChE-positive nerve near the resistance vessels into RChE-positive nerves and BuChE (non-specific ChE)-positive nerves and their terminals. The fomers take Ach for a neurotransmitter or when it is exciting they need Ach. The latters are numerous BuChE-positive nerves and their free nerve endings, but their neurotransmitter are unknown. Therefore it can be shown that all are free nerve endings, but their functions are diverse.
Department of Morphology, Institute of Acupuncture and Moxibustion, Academy of Traditional Chinese Medicine
91 male albino rats were used to observe ChE-positive nerve in somatic connective tissue and to study the peripheral path of conducting needling sensation relating to the acupuncture analgesia.
Acetylthiocholine or butylthiocholine was used as substrate, iso-OMPA was not added to the medium incubation to show the nerve near the resistance vessel by microscopy. Above of all, we observed some nerves in stretch preparation of subcutis which often accompany with arteriole and venule, and with their terminals branch into reticular glomerate and arborescent nerve endings to terminate in matrix of connective tissue. These nerves are named as ChE-positive nerves.
They are thin unmyelinated nerve fibers. After sympathetic surperior cervical ganglionectomy, they are survival. We have traced their whole course in association with small spinal nerve trunk in connective tissue. Therefore, the ChE-positive nerve is afferent through the somatic nerve.
We applied electro-acupuncture to subcutis of the points mimicking Xinming or/ and Huantiao on the both sides of the intact and the sympathetic ganglionectomy animals. Tissue specimens were taken from the points which elevate the pain threshold obviously during acupuncture analgesia.
The afferent receptor of acupuncture in the excised subcutis surounding the needles was searched by microscopy. The free nerve endings in several forms were discovered primarily. The ChE-positive nerves and their terminal branches were also observed in the group of sympathetic ganglionectomy. So we think that they are the path of conducting needling sensation during acupuncture analgesia.
In sarcolemma, muscular interconnective tissue, capsule of knee joint and skeletal muscle, there are ChE-positive nerve to terminate near the resistance vessels by several form of free nerve endings. According to the description of "Ling Shu", needling points is Huang membrane but not skin, skeleton or muscle. We think that somatic connective tissue might be a part of Huan membrane. In Huan membrane there are the structures conducting needling sensation.
According to the results of consecutive method whether added iso-OMPA or not to medium incubation, we divided the ChE-positive nerve near the resistance vessels into RChE-positive nerves and BuChE (non-specific ChE)-positive nerves and their terminals. The fomers take Ach for a neurotransmitter or when it is exciting they need Ach. The latters are numerous BuChE-positive nerves and their free nerve endings, but their neurotransmitter are unknown. Therefore it can be shown that all are free nerve endings, but their functions are diverse.
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